What is the ImageStream MK II?
in Flow Core, is distinctive for
blending speed and sensitivity of a flow tool with the detailed imaging capabilities of a microscope. This combination allows the capture of high-quality images of cells during flow experiments. The ImageStream MKII can analyze up to 5000 cells per second, capturing images of thousands to millions of individual cells in a sample. This speed and accuracy are better than what's usually achieved with regular microscopy methods. Unlike other instruments in the Flow Core, the ImageStream MKII can recover and return unused sample from its fluidic system back into the original sample tube for reuse.
The ImageStream MK II housed in the Flow Core utilizes a 3-laser array, allowing for 6 high-resolution imaging channels including the brightfield and side scatter. Please refer to the table below for available lasers and suggested fluorophores.
The ImageStream MK II is available for individual use following an initial training session. include Assisted Acquisition, Unassisted Acquisition (after training), Flow-Jo data reanalysis, and Assisted Flow-Jo data reanalysis.
Responsibilities and policies
At Flow Core, we don't take responsibility for the data from your experiments. It's up to you to save and keep your data on suitable storage devices like flash drives or hard drives. Remember, the data on the ImageStream workstation is cleaned up regularly. If you haven't saved your data elsewhere, it will be lost.
Flow Core personnel are responsible for the startup and shutdown of this instrument, but users are responsible for arriving at the date/time of their reserved time slot, and are expected to have completed all work by 5:00pm. Please plan out your experiments accordingly.
Contact Us
Macon & Joan Brock Virginia Health Sciences at Old Dominion 91短视频
Flow Cytometry Core Facility
Lewis Hall, Room 3052 and Room 3057
700 W. Olney Road
Norfolk, VA 23507
FlowFacility@EVMS.EDU
Flow Core (LH, Room 3057): 757.446.7435
Flow Cytometry Operator
Alex Coleman
colemaka@EVMS.EDU
Director of Flow Cytometry Core Facilities
Elena Galkina, PhD, FAHA
Email: galkinev@EVMS.EDU
Galkina Lab (LH, Room 3143): 757.446.5761
Galkina Lab (LH, Room 3141): 757.446.5177
Acknowledgments
We thank the Flow Cytometry Core for the excellent technical assistance.
